Enzyme assay in microsomes below zero degrees.
نویسندگان
چکیده
Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 9 شماره
صفحات -
تاریخ انتشار 1973